Upcoming Event

Plant Chromosome Preparation & FISH Workshop

May 14, 2012–May 18, 2012

Organized by:

May 14 Monday  
09:00 – 09:15 Welcome address by Dr. Tom Byram, Director, Western Gulf Forest Tree Improvement Program, Texas Forest Service & Texas A&M
09:15 – 10:00 FISH presentation (Emphasis on proper plant chromosome spreading)
10:10 – 11:00 Root process for enzymatic digestion
11:00 – 12:30 Root collection – Greenhouse
12:30 – 13:30 Lunch (on your own)
13:30 – 18:00 RNase Pre-treatment for FISH Fixation root tips Root processing – Enzymatic digestion – Slide preparation
18:00 – 19:30 Dinner on your own
19:30 – 22:00 Video Demo: Metafer (MetaSystems) – Automated detection of metaphase spreads & karyotyping
   
May 15 Tuesday  
08:30 – 17:30 Practice on your own: Process roots Enzymatic digestion Slide preparation
9:00 – 10:00 FISH discussion – Methodology & Unforeseen problems
12:30 – 13:30 Lunch (on your own)
13:30 – 17:30 FISH Day – 1
Hybridization mixture preparation
Slide Denaturation (Chromosomal DNA)
Hybridization (DNA to DNA)
Overnight incubation
Set-up for hybridization site detection (FISH Day -2)
   
May 16 Wednesday  
08:00 – 11:30 FISH Day – 2
Washing & Hybridization site(s) detection
11:30 – 12:30 Discussion: FISH with BAC, Transgene and genomic DNA probes; Pachytene FISH
12:30 – 13:30 Lunch (on your own)
13:30 – 18:00 Operation of epi-fluorescence microscope and use of MetaSystems FISH analysis software. We have two (State-of-the-Art)) epi-fluorescence microscopes (automated and manual) and will provide training for the both
   
May 17 Thursday  
08:00 – 17:00 Nick-Translation – Cloned DNA
Check Labeled DNA on gel
View FISH slides, capture and process FISH images
12:30 – 13:30 Lunch (on your own)
May 18 Friday  
08:00 – 12:30 FISH slide analysis – Microscope
Metafer (MetaSystems Group Inc.) – automated detection of metaphase spreads
12:30 – 13:30 Lunch (on your own)
13:30 – 16:00 Capture & process FISH images
16:00 – 17:30 Question & Answer Session
Adjourn

PS:
Participants will be encouraged to submit a short report on the following headings within a week from May 18, 2012. Plant somatic chromosome preparation (root tip collection, pretreatment, fixation, enzymatic digestion and chromosome spread) and FISH (probe DNA, probe labeling and detection, RNase-A treatment, hybridization mixture, denaturation, hybridization and detection, capturing and pressing FISH image. The report should include two/three FISH images). For additional details please contact the workshop organizer.

E-mail: nfaridi@tamu.edu; telephone: 979-862-3908 (office), 979-845-2974 (lab).