DNA fingerprinting sets for four southern pines
DNA markers can provide valuable genetic information for forest tree research, breeding, conservation, and restoration programs. When properly evaluated, selected sets of DNA markers can be used to efficiently get information about genetic diversity in regions, forests, or stands, or in seed lots and orchards. Selected markers also can be used to determine parentage or verify clonal identity of individual trees in tree improvement programs and seed production orchards. With these purposes in mind, we developed sets of informative markers for four species of southern pines: shortleaf pine (Pinus echinata Mill.), slash pine (P. elliottii Englem.), longleaf pine (P. palustris Mill.), and loblolly pine (P. taeda L.). We selected 38 markers for all 4 species and then selected markers within each species: 9 for shortleaf pine, 16 for slash pine, 10 for longleaf pine, and 11 for loblolly pine. All markers were originally developed for use in loblolly pine. Once we optimized marker sets for each species, however, there were few markers left in common between the different marker sets. In this report, we provide all the methods and information needed for any molecular biology lab to use the markers in any of the four pine species.