Detection of Phytophthora cinnamomi in forest soils by PCR on DNA extracted from leaf disc baits


Phytophthora cinnamomi Rands causes root rot in a number of important forest tree species around the world, including American chestnut (Castanea dentata) and shortleaf pine (Pinus echinata). Conventional methods for detecting P. cinnamomi in forest soils may require too much time and space to permit widescale and long-term screening of the large sample numbers required for landscape-scale distribution analysis. This project compared conventional detection methods (baiting with full rhododendron leaves or leaf discs and subsequent culturing on selective media) with molecular detection method using DNA extracted from leaf baits. These methods were comparable, and the DNA-based method was correlated with culture-based methods. In a field-validation screening using the leaf bait polymerase chain reaction methodm P. cinnamomi was found across a range of topographic conditions, including dry ridge-top sites and moist lowland sites. Soil texture analysis supports the traditional association of P. cinnamomi with fine-textured soils. Further lage-scale surveys are necessary to elucidate landscape-scale distribution patterns in eastern US forests.

  • Citation: Sena, Kenton; Dreaden, Tyler J.; Crocker, Ellen; Barton, Chris. 2018. Detection of Phytophthora cinnamomi in forest soils by PCR on DNA extracted from leaf disc baits . Plant Health Progress. 19(3): 193-200. 8 p.
  • Posted Date: October 24, 2018
  • Modified Date: October 29, 2018
  • Requesting Print Publications

    Publication requests are subject to availability. Fiscal responsibility limits the hardcopies of publications we produce and distribute. Electronic versions of publications may be downloaded, distributed and printed.

    Please make any requests at

    Publication Notes

    • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
    • Our online publications are scanned and captured using Adobe Acrobat. During the capture process some typographical errors may occur. Please contact the SRS webmaster if you notice any errors which make this publication unusable.
    • To view this article, download the latest version of Adobe Acrobat Reader.