Metagenomic Characterization of Soil Microbial Communities in the Luquillo Experimental Forest (Puerto Rico) and Implications for Nitrogen Cycling

  • Authors: Karthikeyan, Smruthi; Orellana, Luis H.; Johnston, Eric R.; Hatt, Janet K.; Löffler, Frank E.; Ayala-del-Río, Héctor L.; González, Grizelle; Konstantinidis, Konstantinos T.
  • Publication Year: 2021
  • Publication Series: Scientific Journal (JRNL)
  • Source: Applied and Environmental Microbiology
  • DOI: 10.1128/AEM.00546-21

Abstract

The phylogenetic and functional diversity of microbial communities in tropical rainforests, and how these differ from temperate communities remain poorly described but are directly related to the increased fluxes of greenhouse gases such as nitrous oxide (N2O) from the tropics. Towards closing these knowledge gaps, we analyzed replicated shotgun metagenomes representing distinct life zones from four locations in the Luquillo Experimental Forest (LEF), Puerto Rico. These soils had a distinct microbial community composition and lower species diversity when compared to temperate grasslands or agricultural soils. Unlike temperate soils, LEF soils showed little stratification with depth in the first 0-30cm, with ~45% of community composition differences explained solely by location. The relative abundances and nucleotide sequences of N2O reductases (nosZ) were highly similar between tropical forest and temperate soils. However, respiratory NO reductase (norB) was 2-fold more abundant in the tropical soils, which might be relatable to their greater N2O emissions. Nitrogen fixation (nifH) also showed higher relative abundance in rainforest compared to temperate soils (20% vs. 0.1-0.3% of bacterial genomes in each soil type harbored the gene, respectively). Collectively, these results advance our understanding of spatial diversity and metabolic repertoire of tropical rainforest soil communities, and should facilitate future ecological modeling efforts.

  • Citation: Karthikeyan, Smruthi; Orellana, Luis H.; Johnston, Eric R.; Hatt, Janet K.; Löffler, Frank E.; Ayala-del-Río, Héctor L.; González, Grizelle; Konstantinidis, Konstantinos T. 2021. Metagenomic Characterization of Soil Microbial Communities in the Luquillo Experimental Forest (Puerto Rico) and Implications for Nitrogen Cycling. Applied and Environmental Microbiology. 87(12): 43-. https://doi.org/10.1128/AEM.00546-21.
  • Keywords: tropical rainforests, greenhouse gases, Luquillo Experimental Forest, Puerto Rico, soils.
  • Posted Date: September 24, 2020
  • Modified Date: June 17, 2021
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