Field performance o in vitro propagated white ash microplants
This article is part of a larger document. View the larger document here.Abstract
White ash (Fraxinus americana L.) can routinely be propagated by in vitro axillary shoot proliferation and in vitro rooting of microshoots; however, no reports exist on performance and clonal variation following field planting of microplants. To obtain preliminary estimates of among and within clonal variations, we established a small planting with twelve white ash clones in 1992. Ten non-stratified seed from fifteen individual tree collections were cut and germinated in vitro on a medium consisting of Murashige and Skoog (MS) salts and organics, thidiazuron, 6-benzyladenine, indole-3-butyric acid (IBA), 2 to 3 percent sucrose, and 0.7 percent agar. In vitro germinants exhibiting high axillary shoot proliferation rates were repeatedly subcultured to produce microshoots for in vitro rooting experiments.