Skip to main content
U.S. flag

An official website of the United States government

Field performance o in vitro propagated white ash microplants

Informally Refereed

Abstract

White ash (Fraxinus americana L.) can routinely be propagated by in vitro axillary shoot proliferation and in vitro rooting of microshoots; however, no reports exist on performance and clonal variation following field planting of microplants. To obtain preliminary estimates of among and within clonal variations, we established a small planting with twelve white ash clones in 1992. Ten non-stratified seed from fifteen individual tree collections were cut and germinated in vitro on a medium consisting of Murashige and Skoog (MS) salts and organics, thidiazuron, 6-benzyladenine, indole-3-butyric acid (IBA), 2 to 3 percent sucrose, and 0.7 percent agar. In vitro germinants exhibiting high axillary shoot proliferation rates were repeatedly subcultured to produce microshoots for in vitro rooting experiments.

Parent Publication

Citation

Van Sambeek, J.W.;  Preece, John E.; Zaczek, James J. 1999. Field performance o in vitro propagated white ash microplants. In: Stringer, Jeffrey W.; Loftis, David L., eds. 1999. Proceedings, 12th central hardwood forest conference; 1999 February 28-March 1-2; Lexington, KY. Gen. Tech. Rep. SRS-24. Asheville, NC: U.S. Department of Agriculture, Forest Service, Southern Research Station. 293 p
https://www.fs.usda.gov/research/treesearch/65898